Nature

Conserved cell types with divergent features in human versus mouse cortex

Rebecca Hodge, Trygve E. Bakken, Jeremy Miller, Kimberly Smith, Eliza Barkan, Lucas Graybuck, Jennie Close, Brian Long, Osnat Penn, Zizhen Yao, Jeroen Eggermont, Thomas Höllt, Boaz Levi, Soraya Shehata, Brian Aevermann, Allison Beller, Darren Bertagnolli, Krissy Brouner, Tamara Casper, Charles Cobbs, Rachel Dalley, Nick Dee, Song-Lin Ding, Richard Ellenbogen, Olivia Fong, Emma Garren, Jeff Goldy, Ryder Gwinn, Daniel Hirschstein, Dirk Keene, Mohamed Keshk, Andrew Ko, Kanan Lathia, Ahmed Mahfouz, Zoe Maltzer, Medea McGraw, Thuc Nghi Nguyen, Julie Nyhus, Jeffrey Ojemann, Aaron Oldre, Sheana Parry, Shannon Reynolds, Christine Rimorin, Nadiya Shapovalova, Saroja Somasundaram, Aaron Szafer, Elliot Thomsen, Michael Tieu, Richard Scheuermann, Rafael Yuste, Susan Sunkin, Boudewijn P. F. Lelieveldt, David Feng, Lydia Ng, Amy Bernard, Michael Hawrylycz, John Phillips, Bosiljka Tasic, Hongkui Zeng, Allan Jones, Christof Koch, and Ed S. Lein

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Elucidating the cellular architecture of the human neocortex is central to understanding our cognitive abilities and susceptibility to disease.Here we applied single nucleus RNA-sequencing to perform a comprehensive analysis of cell types in the middle temporal gyrus of human cerebral cortex. We identify a highly diverse set ofexcitatory and inhibitory neuronal types that are mostly sparse, with excitatory types being less layer-restricted than expected. Comparison to a similar mouse cortex single cell RNA-sequencing dataset revealed a surprisingly well-conserved cellular architecture that enables matching of homologous types and predictions of human cell type properties. Despite this general conservation, we also find extensive differences between homologous human and mouse cell types, including dramatic alterations in proportions, laminar distributions, gene expression, and morphology. These species-specific features emphasize the importance of directly studying human brain.

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@article{bib:hodge:2019,
    author       = { Hodge, Rebecca and Bakken, Trygve E. and Miller, Jeremy and Smith, Kimberly and Barkan, Eliza and Graybuck, Lucas and Close, Jennie and Long, Brian and Penn, Osnat and Yao, Zizhen and Eggermont, Jeroen and Höllt, Thomas and Levi, Boaz and Shehata, Soraya and Aevermann, Brian and Beller, Allison and Bertagnolli, Darren and Brouner, Krissy and Casper, Tamara and Cobbs, Charles and Dalley, Rachel and Dee, Nick and Ding, Song-Lin and Ellenbogen, Richard and Fong, Olivia and Garren, Emma and Goldy, Jeff and Gwinn, Ryder and Hirschstein, Daniel and Keene, Dirk and Keshk, Mohamed and Ko, Andrew and Lathia, Kanan and Mahfouz, Ahmed and Maltzer, Zoe and McGraw, Medea and Nguyen, Thuc Nghi and Nyhus, Julie and Ojemann, Jeffrey and Oldre, Aaron and Parry, Sheana and Reynolds, Shannon and Rimorin, Christine and Shapovalova, Nadiya and Somasundaram, Saroja and Szafer, Aaron and Thomsen, Elliot and Tieu, Michael and Scheuermann, Richard and Yuste, Rafael and Sunkin, Susan and Lelieveldt, Boudewijn P. F. and Feng, David and Ng, Lydia and Bernard, Amy and Hawrylycz, Michael and Phillips, John and Tasic, Bosiljka and Zeng, Hongkui and Jones, Allan and Koch, Christof and Lein, Ed S. },    
    title        = { Conserved cell types with divergent features in human versus mouse cortex },
    journal      = { Nature },
    volume       = { 573 },
    year         = { 2019 },
    pages        = { 61--68 },
    doi          = { 10.1038/s41586-019-1506-7 },
    pubmedid     = { 31435019 },
    url          = { https://publications.graphics.tudelft.nl/papers/346 },
}